国产综合精品日本亚洲777,国产av无码专区亚洲草草,色一情一乱一伦一区二区三区 ,国产第一色,国产精品视频久久久久,日韩大片高清播放器,国产亚洲精品久久yy50,亚洲精品久久久久久一区二区
您好,歡迎進入研域(上海)化學試劑有限公司網站!
一鍵分享網站到:
  • 技術文章ARTICLE

    您當前的位置:首頁 > 技術文章 > 心肌營養素1(CT1)ELISA試劑盒英文說明書

    心肌營養素1(CT1)ELISA試劑盒英文說明書

    發布時間: 2017-05-21  點擊次數: 1480次

    心肌營養素1(CT1)ELISA試劑盒
    適用生物 Homo sapiens (Human,人)
    檢測范圍 15.63-1000pg/mL 靈敏度 5.5pg/mL
    樣本類型 Serum, plasma and other biological fluids.
    實驗時長 4.5h 實驗方法 雙抗夾心法 

    心肌營養素1(CT1)ELISA試劑盒    規格 96T
    ELISA Kit for Cardiotrophin 1 (CT1)
    FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
    Organism species Homo sapiens (Human)
    Product No. SEA810Hu
    Sample type Serum, plasma and other biological fluids.

    心肌營養素1(CT1)ELISA試劑盒    Format 96-well strip plate
    Assay length 4.5 hours
    Detection range 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL 
    Sensitivity The minimum detectable dose of this kit is typically less than 5.5pg/mL.
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Cardiotrophin 1 (CT1).
    No significant cross-reactivity or interference between Cardiotrophin 1 (CT1) and analogues was observed. Recovery
    Matrices listed below were spiked with certain level of recombinant Cardiotrophin 1 (CT1) and the recovery rates were calculated by comparing the measured value to the expected amount of Cardiotrophin 1 (CT1) in samples.
    Matrix Recovery range (%) Average(%)
    serum(n=5) 82-103 101
    EDTA plasma(n=5) 95-102 99
    heparin plasma(n=5) 85-99 93
    Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    心肌營養素1(CT1)ELISA試劑盒     Linearity
    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cardiotrophin 1 (CT1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Sample 1:2 1:4 1:8 1:16
    serum(n=5) 81-88% 81-92% 96-104% 78-104%
    EDTA plasma(n=5) 78-97% 93-105% 78-97% 84-92%
    heparin plasma(n=5) 94-105% 78-102% 88-102% 79-101%
    Stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Reagents and materials provided
    Reagents Quantity Reagents Quantity
    Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
    Standard 2 Standard Diluent 1×20mL
    Detection Reagent A 1×120μL Assay Diluent A 1×12mL
    Detection Reagent B 1×120μL Assay Diluent B 1×12mL
    TMB Substrate 1×9mL Stop Solution 1×6mL
    Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
    Assay procedure summary
    1. Prepare all reagents, samples and standards;
    2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
    4. Aspirate and wash 3 times;
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
    6. Aspirate and wash 5 times;
    7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
    8. Add 50μL Stop Solution. Read at 450nm immediay.

    心肌營養素1(CT1)ELISA試劑盒    Test principle
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin 1 (CT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cardiotrophin 1 (CT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cardiotrophin 1 (CT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cardiotrophin 1 (CT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

產品中心 Products
在線客服 聯系方式

服務熱線

021-54479081
021-54461587

无码熟妇人妻av在线影片最多| 日本熟妇人妻右手影院| 亚洲综合极品嫩模喷大量白浆| 精品国产亚洲一区二区在线3d| 亚洲人成在线播放网站| 夜夜影院未满十八勿进| 国产亚洲一区二区手机在线观看| 国产精品无码翘臀在线观看| 成人午夜精品无码区| 久久精品视频在线看99| 无码色av一二区在线播放| 人妻少妇边接电话边娇喘| av无码天堂一区二区三区| 国精产品999一区二区三区有限| 男ji大巴进入女人的视频| 久久精品国产99精品国产亚洲性色 | 成av人片在线观看www| 人禽杂交18禁网站免费| 欧美成人精品福利在线视频| 国产精品流白浆无遮挡 | 三上悠亚的av片在线无码| v一区无码内射国产| 日本免费一区二区三区在线播放| 欧美又粗又大xxxxbbbb疯狂 | 亚洲V在线激情| 中文字幕亚洲无线码高清| 丰满少妇被猛烈进入流水| 特级a毛片免费观看| 亚洲一级免费毛片| 亚洲一区二区约美女探花| 成人福利在线视频免费观看| 色综合手机在线| 中文字幕伦视频| 色婷婷电影网| 亚洲中文字幕综合在线| 亚洲一区二区高清在线| 波多野结衣久久高清免费 | 久久久亚洲精品一区二区三区浴池| 成人精品免费视频在线观看| 使劲快高潮了国语对白在线 | 大战熟女丰满人妻av|